Antibiotic Susceptibility and Biofilm Formation of Clinical Isolates of Pseudomonas Species from Wounds Specimens

Abstract

Purpose:  The aim of the study is to investigate biofilm forming capacity and the antibiotic susceptibility profile of Pseudomonas aeruginosa strains isolated from clinical wound specimen. Method: A total number of 60 wound specimens were submitted to the bacteriology laboratory of Abubakar Tafawa Balewa University Teaching Hospital for investigation, and screened for Pseudomonas aeruginosa. The strains were identified on the basis of cultural characteristics, Gram staining, biochemical tests such as citrate, urease, indole, fermentation of sugar using triple sugar agar. The biofilm forming capacity of the strains are tested using the test tube method after standardizing the strains to approximately standard inoculated into a cooked meat broth. The growth rate of Pseudomonas aeruginosa clinical strains after 48 hours incubation are measured by taking the absorbance using Densi-Check. The strain growth rate is also checked. Biofilm formation at the liquid interface (pellicle) is qualitatively scored from the first to the last strain. The clinical significance of the Pseudomonas aeruginosa biofilm forming capacity and resistance to antibiotics which could result to none healing, delayed healing, foul smell of wound infection are checked for the experiment. Results: The analysis of the study shows that the strains are more susceptible to Ciprofloxacin and Streptomycin while the strains are less susceptible to Orfloxacin and Gentamycin. Conclusion: the data derived from human clinical studies make clear that biofilm have an important adverse effect on wound healing. Despite this, more fundamental scientific studies are required to understand what biofilm do to normal wound healing processes from cellular and immunological perspective.