Fotoakustik Mikroskopi Doku Görüntülerinde Pigment Sınırı ve Derinlik Belirleme Tekniği Pigment Boundary and Depth Determination Technique for Photoacoustic Microscopy Image of Tissue

Abstract

Photoacoustic microscopy is a new medical imaging technique that has begun to be used for imaging skin tissue. The tissue viewed in photoacoustic microscopy is divided into pixels and the photoacoustic signal from each pixel is displayed, and depth information cannot be observed in these images. In this study, in order to determine the depths of the pigments from the data obtained by photoacoustic microscopy of the pigmented regions in the three-layer skin environment, a method was developed that first determines the boundaries of the pigmented regions. Photoacoustic microscopy data were obtained by making measurements from the skin model produced by placing inks in a three-layer PDMS phantom in a laboratory environment. Depth information, location and boundary information of the pigmented regions were determined from the image obtained by interpreting the maximum amplitude photoacoustic signal value and the time information of this value with the gradient calculation.