Effect of Genotypes and Sugar Type on Callus Formation and Plant Regeneration Derived from Mature Grains of Egyptian Rice (Oryza sativa L.)Varieties

Journal of King Abdulaziz University-meteorology, Environment and Arid Land Agriculture Sciences Pub Date : 2015-07-22 DOI:10.4197/met.26-2.1

M. El-Ghany

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Abstract

The main objective of the present study was to determine the highest regenerable Egyptian rice cultivars and the best source of carbohydrates. Three medium protocols had been used to determine the in vitro culture response of mature dehusked grains in five Egyption rice cultivars. Factorial experiment in CRD design with ten replications was used to analyze the data. The obtained results showed that in vitro traits were highly significantly affected by genotypes and the interaction between medium protocols and genotypes. Whereas, medium protocols had different effects on the in vitro traits. The results also indicated that the shoot formation (%) and number of shoots per callus traits were highly significantly affected by genotypes, medium protocols and the interaction between medium protocols and genotypes. In conclusion, the cultivars, Sakha 103 and Sakha 104 could be successfully utilized in breeding programs for biotic and abiotic stress tolerance at the cellular level, because of their high frequency of shoot formation on the medium protocol C.

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基因型和糖型对埃及水稻成熟籽粒愈伤组织形成和植株再生的影响品种

本研究的主要目的是确定最高可再生埃及稻品种和最佳碳水化合物来源。采用三种培养基方案测定了5个埃及水稻品种成熟脱壳籽粒的离体培养反应。采用10个重复的CRD设计的析因试验对数据进行分析。结果表明，基因型和培养基方案与基因型之间的互作对植株的体外性状有显著影响。不同培养基处理对离体性状有不同的影响。结果还表明，基因型、培养基方案以及培养基方案与基因型的交互作用对愈伤组织的成苗率和每愈伤组织的芽数均有极显著影响。综上所述，Sakha 103和Sakha 104在培养基方案C上形成芽的频率较高，可成功用于细胞水平上的生物和非生物抗逆性育种计划。

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Journal of King Abdulaziz University-meteorology, Environment and Arid Land Agriculture Sciences

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