Screening of wheat (Ttiticum aestivum L.) cultivars for terminal heat stress tolerance by using biochemical pointer

W. Ahmad, N. Hussain, S. Shah
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Abstract

Among abiotic factors, high temperature is severe constraint limiting crop production. Wheat is a major cereal and staple crop of Pakistan. An experiment was conducted (winter season 2018-19) to expose the effect of warmness induced biochemical alteration on morphological points of Pakistani wheat cultivars. Randomized complete block design (RCBD) with split plot arrangement having three replications was used to experiment. Heat stress treatments H0 = No heat imposition, H1 = heat imposition from (Feekes Scale = 10 to 10.5) booting to complete heading were randomized in main plots while varieties in subplots. Galaxy-2013, Gold-2016, Ghazi-2016, Aas-2011, Johar-2016, Pakistan-2013, F-8, Sahar-2006, Jauher-2016 and AARI-2011. Under heat stress, a smaller reduction in chlorophyll fillings and improvement in antioxidant activities were showed by Ghazi-2019, Aas-2011 and Jhor-2016. Among all other cultivars, higher chlorophyll deprivation and depletion in antioxidant activities under heat stress over control was observed. Reasonably lesser grain filling rate, higher duration, number of grains per spike and yield was detected for cultivars Ghazi-2019, Aas-2011, Jhor-2016 and F-8 under heat stress over control. Convincingly, based on biochemical response and morphological indicators genotypes Ghazi-2019, Aas-2011 and Jhor-2016 manifested heat tolerance. Genotype Fareed-2006 and F-8 manifested medium tolerance. Whereas, genotype AARI-2011, Galaxy-2013, Gold- 2016, Jhor-2016, Pakistain-2013, Sher-2006 and Ujala depicted susceptibility to terminal heat stress.
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利用生化指标筛选耐末端热胁迫小麦品种
在非生物因素中,高温是制约作物生产的严重制约因素。小麦是巴基斯坦的主要谷物和主要作物。本试验于2018- 2019冬季进行,旨在揭示温暖诱导的生化变化对巴基斯坦小麦品种形态点的影响。试验采用随机完全区组设计(RCBD),分为3个重复。热应激处理H0 =不施热,H1 =施热(Feekes量表= 10 ~ 10.5),从孕穗期到完成抽穗期在主区随机,品种在次区随机。Galaxy-2013、Gold-2016、Ghazi-2016、Aas-2011、Johar-2016、巴基斯坦-2013、F-8、撒哈拉-2006、Jauher-2016和AARI-2011。热胁迫下,Ghazi-2019、Aas-2011和Jhor-2016的叶绿素填充量减少幅度较小,抗氧化活性有所提高。在热胁迫下,所有品种的叶绿素剥夺和抗氧化活性均高于对照。热胁迫处理下,Ghazi-2019、Aas-2011、jhar -2016和F-8的籽粒灌浆率较低,生育期、单穗粒数和产量较高。令人信服的是,基于生化反应和形态指标,基因型Ghazi-2019、Aas-2011和Jhor-2016表现出耐热性。基因型Fareed-2006和F-8表现出中等耐受性。而基因型AARI-2011、Galaxy-2013、Gold- 2016、Jhor-2016、pakistan -2013、her-2006和Ujala则表现出对终端热应激的易感性。
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