{"title":"The fluorescence study of the quenching of nanoemulsion by protoporphyrin IX (PPIX)","authors":"Maurice O Iwunze","doi":"10.15406/mseij.2022.06.00185","DOIUrl":null,"url":null,"abstract":"Nanoemulsion is a unique and versatile fluid that solubilizes both ionic and non-ionic molecules. It has been used extensively for drug delivery. Nanoemulsion, prepared with water-oil-surfactant and co-surfactant, is fluorescent at or the near IR region of electromagnetic spectrum. However, when protoporphyrin (PPIX), a useful photosensitizer in PDT modality, and a poorly water-soluble compound, is introduced into the nanoemulsion medium, the observed fluorescence of the nanoemulsion is quenched. We have used the steady-state fluorescence technique to study the mechanism of this quenching. It is observed that the interaction between nanoemulsion and PPIX is 1:1 and the interaction constant, Ka, is about 2.37 x 105/mol and the Free energy of interactions, Ga, is -30.66 kJ/mol.","PeriodicalId":435904,"journal":{"name":"Material Science & Engineering International Journal","volume":"32 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Material Science & Engineering International Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15406/mseij.2022.06.00185","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Nanoemulsion is a unique and versatile fluid that solubilizes both ionic and non-ionic molecules. It has been used extensively for drug delivery. Nanoemulsion, prepared with water-oil-surfactant and co-surfactant, is fluorescent at or the near IR region of electromagnetic spectrum. However, when protoporphyrin (PPIX), a useful photosensitizer in PDT modality, and a poorly water-soluble compound, is introduced into the nanoemulsion medium, the observed fluorescence of the nanoemulsion is quenched. We have used the steady-state fluorescence technique to study the mechanism of this quenching. It is observed that the interaction between nanoemulsion and PPIX is 1:1 and the interaction constant, Ka, is about 2.37 x 105/mol and the Free energy of interactions, Ga, is -30.66 kJ/mol.