{"title":"Determination of Bioactive Compounds by Highly Sensitive Electrochemical Detection in Liquid Chromatography","authors":"Akira Kotani","doi":"10.5189/revpolarography.66.23","DOIUrl":null,"url":null,"abstract":"Determinations of bioactive compounds were carried out by various electrochemical detections (ECDs) hyphenated with high-performance liquid chromatography (HPLC-ECD) systems. An HPLC-ECD using a capillary column (0.2 mm i.d.) achieved determinations of catechins at femtogram levels. A three-channels HPLC-ECD using a column switching technique provided to the simultaneous determination of caffeoylquinic acids and flavonoids in Chrysanthemum morifolium flowers. HPLC-ECD systems by the means of reduction of 3,5-di- tert -butyl-1,2-benzoquinone and oxidation of trolox were developed to determine polyunsaturated fatty acids and theophylline in plasma, respectively. These HPLC-ECD systems with sensitivity and precision produced a practically useful method for the analyses of biological and herbal medicine samples.","PeriodicalId":305513,"journal":{"name":"Review of Polarography","volume":"23 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2020-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Review of Polarography","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5189/revpolarography.66.23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Determinations of bioactive compounds were carried out by various electrochemical detections (ECDs) hyphenated with high-performance liquid chromatography (HPLC-ECD) systems. An HPLC-ECD using a capillary column (0.2 mm i.d.) achieved determinations of catechins at femtogram levels. A three-channels HPLC-ECD using a column switching technique provided to the simultaneous determination of caffeoylquinic acids and flavonoids in Chrysanthemum morifolium flowers. HPLC-ECD systems by the means of reduction of 3,5-di- tert -butyl-1,2-benzoquinone and oxidation of trolox were developed to determine polyunsaturated fatty acids and theophylline in plasma, respectively. These HPLC-ECD systems with sensitivity and precision produced a practically useful method for the analyses of biological and herbal medicine samples.
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