A convenient method for regional monoamine oxidase-a determination by [14C]clorgyline autoradiography

International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology Pub Date : 1992-08-01 DOI:10.1016/0883-2897(92)90096-H

Matsutaro Murakami , Yasushi Kondoh , Yin Weimin , Sigenori Mizusawa , Hiroyuki Nakamichi , Kazuhiro Takahashi , Hiroshi Sasaki , Hidehiro Iida , Shuichi Miura , Iwao Kanno , Kazuo Uemura

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引用次数: 4

Abstract

The availability of clorgyline for regional monoamine oxidase-A (MAO-A) determination was examined using [¹⁴C]clorgyline in rat. [¹⁴C]Clorgyline was synthesized by the methylation reaction of N-desmethylclorgyline and [¹⁴C]methyliodide in dimethylformamide with high radiochemical yield. The MAO-A distribution map by autoradiography correlated with that by histochemical technique and its quantity was consistent with the calculated MAO-A amount based on previous reports. The combination of labeled clorgyline and autoradiographic technique will promise the quantitative measurement of regional MAO-A distribution.

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