Isolation of pathogenic bacteria from stagnant waste water around residential buildings

Abstract

Worldwide water born pathogen contamination and disease arise major issues between the communities. Due to heavy rain most of the places were sink in to the rain water around. In addition, residing peoples were discharge their septic tank and sewage water in to stagnant water. After 2-3 months duration stagnant rain water contaminated by pathogenic microorganism. Such pathogenic organism causes many diseases to the people who are living near such contaminated area. The aim of the investigation was to isolate and identify pathogenic bacteria from stagnant water around residential area. For this study two locations were selected, and contaminated water samples were collected from Mettu theru (L1), and Srinvasapuram( L2). Isolated bacteria from L1 were Chryseobacterium sp., Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa, Escherichia coli, Bacillus megaterium, Enterococcus sp, Streptococcus sp, Serratia marcescens and fro L2 were Staphylococcus aureus, Proteus vulgaris, Bacillus cereus, Providencia rettgeri, Escherichia coli, Serratia marcescens, Chryseobacterium, Pseudomonas aeruginosa, Enterococcus. Pathogenesity of the isolates measured by antibiotic sensitivity test. The sensitivity and resistance profile were unique in each bacterium. Based on the research Ecoli has the highest antibiotic resistance profile against majority of the antibiotics similarly Streptococcus and staphylococcus had maximum resistance. This research concludes that we should avoid stagnant water very near to the residential area, also need to create awareness to the people about the effects contaminated water.