Ice Nucleation Active bacteria in Mount Lawu forest, Indonesia: 2. Identification and characterization of ina gene bacteria isolated from lichens

Abstract

Abstract. Arifin TN, Susilowati A, Sutarno. 2018. Ice Nucleation Active bacteria in Mount Lawu forest, Indonesia: 2. Identification and characterization of ina gene bacteria isolated from lichens. Asian J For 2: 39-46. Ice nucleation active (INA) bacteria can catalyze ice formation. Moreover, these bacteria cause frost injury in plants. This study aimed to determine the ina bacteria species of Mount Lawu forest, Java, Indonesia, based on the 16S rRNA gene and the characters of ice nucleation active gene coding of ina bacteria on lichens used ina gene primer. First, the isolates of ina bacteria were grown in NAG, and the DNA could be isolated. After that, genes coding for 16S rRNA and ina were amplified, and the amplification products were sequenced. Furthermore, the sequences were analyzed with BLAST program to know the similarity of bacteria species and ina gene characters. The result showed that the isolated ina bacteria had similarities with Pantoea, Pseudomonas, and Rahnella. The ina gene from N.2.1.B-13 isolate consists of 189 amino acids, dominated by a common amino acid found in other ina genes, like Alanine, Glycine, Tyrosine, Serine, Leucine, and Threonine. N terminal and C terminal are not found because ina gene from N.2.1.B-13 isolate is similar to the ice nucleation protein gene Pseudomonas borealis from bases 2947 bp to 3491 bp or 871 to 1060 amino acids.