Isolation, Identification and in vitro Screening of Rhizospheric Fungi for Biological Control of Macrophomina phaseolina

Asian Journal of Plant Pathology Pub Date : 2015-04-01 DOI:10.3923/AJPPAJ.2015.175.188

M. Mallikarju, B. Gowdu

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引用次数: 3

Abstract

The present study was aimed to identify charcoal rot pathogen of groundnut (Arachis hypogaea L.) in Anantapur district, Andhra Pradesh and assess the interactions among the fungal isolates from rhizosphere. During field survey, a potential charcoal rot pathogen Macrophomina phaseolina was isolated and identified. About 10 different rhizosphere soils of healthy groundnut plants were selected for isolation of fungi. Among 25 isolates assayed for antagonism, ten different fungi significantly inhibited colony growth of M. phaseolina in dual culture plates and morphological identification was done at (400X). Antagonistic fungi inflicted 25.6-41% mycelial growth inhibition in M. phaseolina. Colony growth and sclerotia production of M. phaseolina ceased effectively by Emericella nidulans and E. rugulosus, respectively. Mutual inhibition between Alternaria alteranata, Aspergillus flavus, A. terreus, Penicillium chrysogenum and M. phaseolina was observed. Penicillium oxalicum, Gliocladium roseum and Aspergillus versicolor significantly reduces growth with <1 mm demarcation lineat 7 and 14 Days after Inoculation (DAI) on PDA medium. These antagonistic interactions influence the growth of the pathogen.

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菜绿巨噬菌根际真菌的分离、鉴定及离体筛选

本研究旨在鉴定安得拉邦Anantapur地区花生(Arachis hypogaea L.)的木炭腐病病原菌，并评估根际真菌分离株之间的相互作用。在野外调查中，分离鉴定了一种潜在的炭腐菌。选取10种不同的健康花生根际土壤进行真菌分离。在25个拮抗菌株中，10种不同的真菌在双培养板上显著抑制菜绿分枝杆菌的菌落生长，并在(400X)下进行形态鉴定。拮抗真菌对菜绿支原体菌丝生长的抑制作用为25.6 ~ 41%。绿豆绿病菌的菌落生长和菌核产生分别被细粒绿芽孢杆菌和细粒绿芽孢杆菌有效抑制。观察到互花霉、黄曲霉、土霉、黄化青霉和菜绿霉之间的相互抑制作用。在PDA培养基上接种后7和14 d，草青霉、玫瑰胶霉和花色曲霉显著抑制生长，分界线<1 mm。这些拮抗相互作用影响病原体的生长。

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Asian Journal of Plant Pathology

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