M. Pinsard, M. Schmeltz, J. Kolk, K. Patten, L. Ramunno, M. Schanne-Klein, F. Légaré
{"title":"Removing artifacts in Second Harmonic Generation imaging by interferometry","authors":"M. Pinsard, M. Schmeltz, J. Kolk, K. Patten, L. Ramunno, M. Schanne-Klein, F. Légaré","doi":"10.1109/PN.2019.8819578","DOIUrl":null,"url":null,"abstract":"We report the use of Interferometric Second Harmonic Generation (I-SHG) microscopy to eliminate imaging artifacts from SHG patterns. Boundaries between two regions of opposite polarities in periodically-poled lithium niobate (PPLN) and in muscle myosin are compared, and shown to produce respectively incoherent contributions to the SHG signal and artefactual interferences. I-SHG allows to remove those artifacts, which opens a great opportunity for a better characterization of SHG images of various materials, especially complex media like biological tissues.","PeriodicalId":448071,"journal":{"name":"2019 Photonics North (PN)","volume":"149 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2019-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2019 Photonics North (PN)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/PN.2019.8819578","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
We report the use of Interferometric Second Harmonic Generation (I-SHG) microscopy to eliminate imaging artifacts from SHG patterns. Boundaries between two regions of opposite polarities in periodically-poled lithium niobate (PPLN) and in muscle myosin are compared, and shown to produce respectively incoherent contributions to the SHG signal and artefactual interferences. I-SHG allows to remove those artifacts, which opens a great opportunity for a better characterization of SHG images of various materials, especially complex media like biological tissues.
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