[Efferent potential-dependent ionic currents developing in frog myoblast culture].

Neirofiziologiia = Neurophysiology Pub Date : 1992-01-01
V I Luk'ianenko, I E Katina, G A Nasledov, A V Lonskiĭ
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Abstract

Variety of outward voltage-dependent currents in cultured frog myoblasts were registered by means of whole cell voltage clamp. Six types of potassium currents (Ik) which reach the peak value (-10 mV) in 5, 12, 20, 30, 50 ms (fast) and do not reach the maximum in 7 s (slow) were established. High sensitivity to low temperature (+5 degrees) was shown for channels of very fast and of slow non-inactivated Ik. The assumption is made that some of Ik--5, 20 and 30 ms to peak are especially characteristic of myoblasts fusion stage.

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[青蛙成肌细胞培养中产生的依赖电位的离子电流]。
用全细胞电压钳法记录了培养的蛙成肌细胞外电压依赖性电流的变化。建立了6种钾电流(Ik),分别在5、12、20、30、50 ms(快速)内达到峰值(-10 mV),在7 s(慢速)内不达到最大值。对低温(+5度)的高灵敏度显示了非常快速和缓慢的非灭活Ik通道。假设Ik- 5、20和30 ms的峰值是成肌细胞融合阶段的特征。
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