{"title":"Assessment of TNF-α Gene Expression in Type 2 Diabetes Patients with Nephropathy","authors":"S. Sharif, Samra Anees, Muhammad Roman, S. Jahan","doi":"10.54393/fbt.v2i02.17","DOIUrl":null,"url":null,"abstract":"Type 2 diabetes mellitus (T2DM) and its complications, including nephropathy, are a significant public health concern worldwide. Tumor necrosis factor-alpha (TNF-α) is a proinflammatory cytokine that plays a crucial role in the development and progression of chronic inflammatory diseases, including T2DM and diabetic nephropathy. Objective: To evaluate of TNF-α gene expression in patients with T2DM and nephropathy compared to healthy individuals. Methods: The cross-sectional study conducted on 120 individuals divided into three groups: healthy individuals, type 2 diabetes mellitus (T2DM) patients without diabetic nephropathy, and T2DM patients with nephropathy. RNA was extracted and TNF-α gene expression was evaluated using PCR and statistical analysis was done using SPSS software. Results: The results showed almost 4.2-fold induced expression of TNF-α in T2DM patients without nephropathy compared to the normal group. Conclusion: The study reports that in diabetic nephropathy patients, Gene expression of gene TNF-a shows increases in cases when compared with healthy subjects","PeriodicalId":114319,"journal":{"name":"Futuristic Biotechnology","volume":"18 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Futuristic Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.54393/fbt.v2i02.17","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
Type 2 diabetes mellitus (T2DM) and its complications, including nephropathy, are a significant public health concern worldwide. Tumor necrosis factor-alpha (TNF-α) is a proinflammatory cytokine that plays a crucial role in the development and progression of chronic inflammatory diseases, including T2DM and diabetic nephropathy. Objective: To evaluate of TNF-α gene expression in patients with T2DM and nephropathy compared to healthy individuals. Methods: The cross-sectional study conducted on 120 individuals divided into three groups: healthy individuals, type 2 diabetes mellitus (T2DM) patients without diabetic nephropathy, and T2DM patients with nephropathy. RNA was extracted and TNF-α gene expression was evaluated using PCR and statistical analysis was done using SPSS software. Results: The results showed almost 4.2-fold induced expression of TNF-α in T2DM patients without nephropathy compared to the normal group. Conclusion: The study reports that in diabetic nephropathy patients, Gene expression of gene TNF-a shows increases in cases when compared with healthy subjects
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