A. Clausina, Angala Andzi Jenny Carmela, Mongo Onkouo Arnaud, Itoua Ngaporo Ngala Akoa, M. Florent, N. Stéphane, N. Marlyse, Adoua Céline Sandra, D. Gassaye, Atipo Ibara Blaise Irénée, Ibara Jean Rosaire
{"title":"Genotypes of The Hepatitis B Virus in Congo-Brazzaville","authors":"A. Clausina, Angala Andzi Jenny Carmela, Mongo Onkouo Arnaud, Itoua Ngaporo Ngala Akoa, M. Florent, N. Stéphane, N. Marlyse, Adoua Céline Sandra, D. Gassaye, Atipo Ibara Blaise Irénée, Ibara Jean Rosaire","doi":"10.33425/2639-9334.1036","DOIUrl":null,"url":null,"abstract":"Introduction: The hepatitis B virus (HBV) is characterized by significant genotypic heterogeneity. Circulating genotypes in Congo are not all known. The purpose of this work is to establish a national genotypic mapping of HBV. Patients and Methods: Descriptive, cross-sectional study carried out in the blood transfusion centers and integrated health centers of the Congo departments, from 1 January 2010 to 31 December 2016. Asymptomatic, blood donor or consulting patients formed the study population. The search for HBsAg was carried out by rapid tests, supplemented by a discriminatory screening test by the ELISA technique ; then detection of the HBV viral DNA by nested PCR on the HBPol region of the PreS1 / PreS2 / HBsAg domains of the S gene, using the HBPr1 / HBPr135 and HBPr2 / HBPr3 primer pairs and determination of the genotypes by sequencing from PCR product. Results: A total of 3017 patients with mean age of 39 +/16 years were included, including 1576 men and 1440 women. Of these, 379 (12.5%) were positive for HBsAg. Of these 379 samples submitted to the molecular study, 321 (84%) were positive for HBV viral DNA. The genotypes found were genotype E (n = 207, 54.6%), A (n = 88, 23.2%), D (n = 4, 1%), B (n = 1, 0.3). %), B / C (n = 1, 0.3%) and C (n = 1, 0.3%). In 19 cases (5%) the genotype could not be determined. The identified subgenotypes were A3 and A6 for genotype A and D7 for genotype D. The distribution of genotypes was almost homogeneous across all departments. Genotype D was only identified in one department, as were genotypes B and C in only one other department. Conclusion: Genotypes E and A are the most common in our country. The presence of genotypes D, B and C is probably related to population migrations. Subgenotypes A3, A6 and D7 were the only ones identified in our work.","PeriodicalId":211573,"journal":{"name":"Gastroenterology, Hepatology & Digestive Disorders","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gastroenterology, Hepatology & Digestive Disorders","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33425/2639-9334.1036","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Introduction: The hepatitis B virus (HBV) is characterized by significant genotypic heterogeneity. Circulating genotypes in Congo are not all known. The purpose of this work is to establish a national genotypic mapping of HBV. Patients and Methods: Descriptive, cross-sectional study carried out in the blood transfusion centers and integrated health centers of the Congo departments, from 1 January 2010 to 31 December 2016. Asymptomatic, blood donor or consulting patients formed the study population. The search for HBsAg was carried out by rapid tests, supplemented by a discriminatory screening test by the ELISA technique ; then detection of the HBV viral DNA by nested PCR on the HBPol region of the PreS1 / PreS2 / HBsAg domains of the S gene, using the HBPr1 / HBPr135 and HBPr2 / HBPr3 primer pairs and determination of the genotypes by sequencing from PCR product. Results: A total of 3017 patients with mean age of 39 +/16 years were included, including 1576 men and 1440 women. Of these, 379 (12.5%) were positive for HBsAg. Of these 379 samples submitted to the molecular study, 321 (84%) were positive for HBV viral DNA. The genotypes found were genotype E (n = 207, 54.6%), A (n = 88, 23.2%), D (n = 4, 1%), B (n = 1, 0.3). %), B / C (n = 1, 0.3%) and C (n = 1, 0.3%). In 19 cases (5%) the genotype could not be determined. The identified subgenotypes were A3 and A6 for genotype A and D7 for genotype D. The distribution of genotypes was almost homogeneous across all departments. Genotype D was only identified in one department, as were genotypes B and C in only one other department. Conclusion: Genotypes E and A are the most common in our country. The presence of genotypes D, B and C is probably related to population migrations. Subgenotypes A3, A6 and D7 were the only ones identified in our work.