IDDF2021-ABS-0106 Significance of the expression of iron death-related gene hsbp1 in esophageal squamous cell carcinoma

Abstract

Background To analyze the prognosis and expression of the ferroptosis-related gene HSBP1 in esophageal squamous cell carcinoma and its possible regulatory mechanisms. Methods Screen ferroptosis-related genes from previously published literature and GSEA (Gene Set Enrichment Analysis) website. We used R 4.0.3 software to perform differential analysis on the expression and prognosis of ferroptosis-related genes, and to evaluate the immune score. Results A total of 30 ferroptosis-related genes were differentially expressed in esophageal squamous cell carcinoma (IDDF2021-ABS-0106 Figure 1, IDDF2021-ABS-0106 Figure 2). Prognostic analysis showed that only HSBP1 was associated with the prognosis of esophageal squamous cell carcinoma, and its high expression indicated a poor prognosis (IDDF2021-ABS-0106 Figure 3). In addition, univariate and multivariate COX regression analysis further proved that high expression of HSBP1 is an independent risk factor affecting prognosis (IDDF2021-ABS-0106 Table 1). GSEA enrichment analysis shows that high HSBP1 may be involved in the regulation of nucleotide excision and repair, spliceosome, proteasome and other pathways (IDDF2021-ABS-0106 Figure 4). The analysis of immune checkpoint results showed that patients with high HSBP1 expression had increased immune checkpoint molecules CLAG3 and TIGIT (IDDF2021-ABS-0106 Figure 5). Conclusions The iron death-related gene HSBP1 may be involved in the occurrence and development of esophageal squamous cell carcinoma and the regulation of immune checkpoints. This study provides a new research direction for future HSBP1-related esophageal squamous cell carcinoma research and treatment targets.