A microfluidic static gradient generator using limited diffusuon through T-shaped narrow channels

M. Tamura, S. Maeda, Tomohisa Ogawa, Masato Tanaka, N. Futai
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引用次数: 1

Abstract

We have developed a poly (dimethylsiloxane) (PDMS)-cast microfluidic chip that contains a thin (cross-sectional area less than 1.0 μm2) but high aspect ratio (1:1 ~ 1:3) microchannels. Experimental results showed that the gradient of an example of test substances (sodium fluorescein) remained constant in the thin channel over a long period of time (30 minutes) by simple manual liquid handling. We aim to develop a thinner channel keeping the gradient longer time.
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一种利用有限扩散通过t形窄通道的微流体静态梯度发生器
我们开发了一种聚二甲基硅氧烷(PDMS)铸造的微流控芯片,它包含一个薄(横截面积小于1.0 μm2)但高宽高比(1:1 ~ 1:3)的微通道。实验结果表明,通过简单的手工液体处理,以荧光素钠为例的测试物质的梯度在长时间(30分钟)内在薄通道中保持恒定。我们的目标是开发一种更薄的通道,使梯度保持更长时间。
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