The Effects of SNEDDS (Self-Nano Emulsifying Drug Delivery System) Black Cumin Oil Seeds (Nigella sativa L) to The Histopatology of Lung Organs of The Sprague Dawley Rats That Induced by DMBA

Abstract

Lung cancer is the cancer that caused death, especially for men in Indonesia. One of the compounds that causes cancer is 712-Dimethylben(a)anthracene (DMBA). Black cumin seed oils (BCSO) is able to reduce the damage of lung cells to the DMBA-induced rat. To improve total medicine that could arrive and react at the cancer cell, it’s necessary to make selfnanoemulsifying drug delivery system (SNEDDS). The aim of this research is to know the effect of SNEDDS BCSO into of histopathological lung organ of the Sprague Dawley (SD) rat that’s already DMBA-induced. The making of SNEDDS is by using BCSO, tween 80 and PEG 400. Treatment was done in vivo towards 25 SD male rats, which are divided to 4 groups. The first group act as the baseline, the second one are given with the DMBA, the third group was given BCSO as equivalent as thymoquinone 6.8 mg/KgBW, and group 4 was given BCSO SNEDDS as equivalent as 6.8 mg/KgBW of thymoquinone. Next, the rat discharged and lung organs are taken then being H & E painted. Based on results of histopathology lung in a descriptive manner, there’s no specific differences of histopathology between the group treatment. Next, scoring is done based on alveolar category damage. After that, was done the Kruskal Wallis test, which obtained P value = 0.801. Because of the p value was bigger than 0.05, then this statistical test didn’t show any significant difference at each of the group. This research concludes that there is no influence from BCSO SNEDDS to histopathology of DMBA-induced lung compared with BCSO administration only. Keywords— SNEDDS, nigella sativa, DMBA, histopathology, lung