[In vitro effects of 1,25-dihydroxycholecalciferol on alkaline phosphatase and gamma-glutamyltransferase activity in hypophysectomized rats].

Archives internationales de physiologie et de biochimie Pub Date : 1990-03-01 DOI:10.3109/13813459009115738

M Prelot, T X Do, P Planchenault, A Girault

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引用次数: 3

Abstract

Effects in vitro of 1,25-dihydroxycholecalciferol (1,25-(OH)2D3) on alkaline phosphatase (PAL), gamma-glutamyltransferase (gamma-GT) and acid phosphatase (PAC) activities were investigated on renal cortex from hypophysectomized rats. In these animals the biosynthesis of 1,25-(OH)2D3 and the specific activities of kidney PAL and gamma-GT were decreased. The course of these effects was determined from 45 min to 8 h. In the presence of 1,25-(OH)2D3 (2 x 10(-6) M) a delayed (5h) but simultaneous stimulation of the three enzymes was observed. It reached a maximum at 6h and disappeared at 8h. The dose-response relation was studied at 6h. In the presence of 1,25-(OH)2D3 (5 x 10(-7) M), the three enzymes were activated. The effect was maximal at 10(-6) M; it was +22% for PAL, +17% and +15% respectively for gamma-GT and PAC compared with controls. Cycloheximide suppressed the induction of PAL but not of gamma-GT activity. The effects of the secosteroid on renal enzymes seems to be a pharmacological more than a physiological one.

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[1,25-二羟基胆骨化醇对去垂体大鼠碱性磷酸酶和γ -谷氨酰转移酶活性的影响]。

研究了1,25-二羟基胆骨化醇(1,25-(OH)2D3)对垂体去骨大鼠肾皮质碱性磷酸酶(PAL)、γ -谷氨酰转移酶(γ - gt)和酸性磷酸酶(PAC)活性的影响。在这些动物中，125 -(OH)2D3的生物合成和肾PAL和γ - gt的比活性降低。这些作用的过程从45分钟到8小时确定。在125 -(OH)2D3 (2 × 10(-6) M)存在的情况下，观察到三种酶的延迟(5h)但同时刺激。6h达到最大值，8h消失。6h时研究剂量-反应关系。在1,25-(OH)2D3 (5 × 10(-7) M)存在下，这三种酶被激活。10(-6) M时效果最大;与对照组相比，PAL、γ - gt和PAC分别为+22%、+17%和+15%。环己亚胺对PAL的诱导有抑制作用，但对γ - gt活性无抑制作用。第二类固醇对肾酶的作用似乎是药理学上的，而不是生理上的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Archives internationales de physiologie et de biochimie

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