[Effect of ultraviolet light on the UV/VIS absorption spectrum of components of Eagle (MEM) cell culture medium].

G Bollmann, K Redmann
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Abstract

The impact of ultraviolet light on uv/vis absorption spectra of selected individual components of the cell breeding medium according to Eagle (MEM) was investigated. The strongest alterations of light absorption were detected in L-phenylalanine, L-tyrosine and L-tryptophan. Thus, the absorption behaviour of the Eagle (MEM) medium changed post radiation may be attributed to spectrophotometric alterations of absorption in aromatic amino acids. The results are discussed with regard to the effect on the surface charge of erythrocytes.

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[紫外光对Eagle (MEM)细胞培养基成分紫外/可见吸收光谱的影响]。
根据Eagle (MEM)研究了紫外光对细胞培养培养基中各组分紫外/可见吸收光谱的影响。l -苯丙氨酸、l -酪氨酸和l -色氨酸的光吸收变化最大。因此,辐射后Eagle (MEM)介质的吸收行为变化可能归因于对芳香氨基酸吸收的分光光度变化。讨论了对红细胞表面电荷的影响。
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