Development of a polyclonal antibody based immunoassay for detection of molybdenum levels in environmental water

2011 3rd International Conference on Awareness Science and Technology (iCAST) Pub Date : 2011-09-01 DOI:10.1109/ICAWST.2011.6163121

Huaguo Huang, Jinqing Jiang, Haitang Zhang, Ziliang Wang, Xin-peng Li

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引用次数: 2

Abstract

An indirect competitive ELISA (icELISA) based on anti-mice polyclonal antibody has been developed for the determination of molybdenum (Mo) levels in environmental water. For this reason, isothiocyanate method was employed to synthesize the artificial antigen of Mo-ITCBE-BSA, and Balb/C mice were used to prepare the polyclonal antibody. Based on the square matrix titration, an icELISA method was developed. The dynamic range was from 0.16 to 78 ng/mL, with LOD and IC50 value of 0.08 ng/mL and 3.2 ng/mL, respectively. Except for cross-reactivity to Co2+ (36.3%) and Hg2+ (42.5%), negligible cross-reactivity to other metal ions was observed. Under optimization dilution folds, the recoveries of Mo2+ were in the range of 95.3–117.5%, 88.7–113.5% and 97.5–104.2% for tap water, river water and lake water, respectively. Therefore, this assay has the potential to be incorporated into a quantitative monitoring program for the rapid screening of molybdenum levels in water.

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基于多克隆抗体的环境水中钼含量检测方法的建立

建立了一种基于抗小鼠多克隆抗体的间接竞争酶联免疫吸附测定法(icELISA)，用于环境水中钼的测定。为此，采用异硫氰酸盐法合成Mo-ITCBE-BSA人工抗原，并利用Balb/C小鼠制备多克隆抗体。在方阵滴定法的基础上，建立了icELISA方法。动态范围为0.16 ~ 78 ng/mL, LOD值为0.08 ng/mL, IC50值为3.2 ng/mL。除对Co2+(36.3%)和Hg2+(42.5%)有交叉反应外，对其他金属离子的交叉反应可忽略不计。优化稀释倍数下，自来水、河水、湖水中Mo2+的回收率分别为95.3 ~ 117.5%、88.7 ~ 113.5%、97.5 ~ 104.2%。因此，该方法有可能被纳入定量监测程序，用于快速筛选水中钼含量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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2011 3rd International Conference on Awareness Science and Technology (iCAST)

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