Importance of Conjunctival Swab Culture and Sensitivity Test Before Cataract Surgery

J. Ahmad
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Abstract

Purpose: To determine the spectrum of infectious-agent from conjunctival swab and antibiotic susceptibility. To observe the necessity of conjunctival swab culture and sensitivity prior to intraocular surgery as a preventive measure against post operative endophthalmitis in the socio-economic perspective of our country. Method: A retrospective review of microbiological records of conjunctival swab was done for 22 months (From August 2000 to May 2002) in BSMMU, Dhaka, Bangladesh. We selected the culture positive cases for our study. Antibiotic susceptibility of these isolates was determined for fourteen ocular and systemic antibiotics. Result: Among 209 attendant cases, 57 were culture positive. All of them were gram positive bacteria. Most of them were staphylococcus epidermis 47 (82.5%) others were staphylococcus aureus 5 (8.77%); streptococcus viridans 5 (8.77%). There was specific age variation also. All organisms were 100% sensitive to Chloramphenicol. Staphylococcus epidermidis, treptococcus viridans were 100% sensitive to Gentamycin and Ciprofloxacin, while staphylococcus aureus show 80% sensitivity. Organisms were least sensitive to Neosporin. Conclusion: Commonest isolated organism from conjunctival swab is staphylococcus epidermidis (82.5%). All the organism shows full range of sensitivity and no resistance to chloramphenicol; also maximum sensitivity and no resistance to Gentamycin, Ciprofloxacin in our study group and from this it can be conclude that instead of doing random C/S for conjunctival swab we can give prophylactic broad spectrum antibiotic eye drop to starilize conjunctival sac before surgery.
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白内障手术前结膜拭子培养及敏感性试验的重要性
目的:测定结膜拭子感染源谱及对抗生素的敏感性。从我国社会经济角度观察眼内手术前结膜拭子培养及敏感性作为预防术后眼内炎的必要性。方法:回顾性分析2000年8月至2002年5月在孟加拉国达卡BSMMU进行的22个月的结膜拭子微生物学记录。我们选择培养阳性案例进行研究。测定了这些分离株对14种眼部和全身抗生素的敏感性。结果:209例患者中培养阳性57例。都是革兰氏阳性菌。其中以表皮葡萄球菌47株(82.5%)居多,金黄色葡萄球菌5株(8.77%);翠绿链球菌5(8.77%)。也有特定的年龄差异。所有微生物对氯霉素100%敏感。表皮葡萄球菌、翠绿链球菌对庆大霉素、环丙沙星的敏感性为100%,金黄色葡萄球菌对环丙沙星的敏感性为80%。微生物对新孢菌素最不敏感。结论:结膜拭子中最常见的分离菌为表皮葡萄球菌(82.5%)。所有生物对氯霉素均表现出全方位的敏感性,无耐药性;我们的研究组对庆大霉素、环丙沙星的敏感性最高且无耐药性,由此可以得出结论,我们可以在手术前给予预防性广谱抗生素滴眼液来消毒结膜囊,而不是对结膜拭子进行随机C/S。
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