{"title":"SRS microscopy as a low-invasive 3D bioimaging technique","authors":"K. Itoh, Y. Ozeki","doi":"10.1109/WIO.2010.5582519","DOIUrl":null,"url":null,"abstract":"We introduce stimulated Raman scattering microscopy which allows us to visualize unstained live cells with high sensitivity and high contrast. The shot-noise limited sensitivity can be achieved based on high-frequency lock-in detection using subharmonically synchronized two-color pulses.","PeriodicalId":201478,"journal":{"name":"2010 9th Euro-American Workshop on Information Optics","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2010-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2010 9th Euro-American Workshop on Information Optics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/WIO.2010.5582519","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
We introduce stimulated Raman scattering microscopy which allows us to visualize unstained live cells with high sensitivity and high contrast. The shot-noise limited sensitivity can be achieved based on high-frequency lock-in detection using subharmonically synchronized two-color pulses.
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