Automated electrical stimulation and physical activity monitoring of zebrafish larvae

2015 IEEE Jordan Conference on Applied Electrical Engineering and Computing Technologies (AEECT) Pub Date : 2015-12-21 DOI:10.1109/AEECT.2015.7360529

Q. Al-Jubouri, W. Al-Nuaimy, M. Al-Taee, J. L. Luna, L. Sneddon

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引用次数: 10

Abstract

Zebrafish (Danio rerio) is accepted as an efficient model to aid in research over a broad range of human diseases and drug discovery. However, obtaining evidences of pain perception similarity between adults and larvae is still a considerable scientific challenge. This paper presents an automated test-bed for electrical stimulation and physical activity monitoring of zebrafish larvae. It comprises of infrared digital camera and 25 test arenas along with the required software and hardware circuitry. Unlike existing systems, the developed system is fully automatic and does not require any manual adjustments. In addition, it is capable of estimating and monitoring physical activity of multiple larvae at various levels of painful stimulation. Performance of the developed testbed is assessed experimentally using various stimulation levels for numerous test batches of zebrafish larvae. The obtained results demonstrated accurate tracking and monitoring of multiple larvae responses for various levels of painful stimulation.

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斑马鱼幼体的自动电刺激和身体活动监测

斑马鱼(Danio rerio)被认为是一种有效的模型，可以帮助研究广泛的人类疾病和药物发现。然而，获得成虫和幼虫之间疼痛感知相似性的证据仍然是一个相当大的科学挑战。本文介绍了一种用于斑马鱼幼体电刺激和生理活动监测的自动化试验台。它由红外数码相机和25个测试场地以及所需的软件和硬件电路组成。与现有系统不同，开发的系统是全自动的，不需要任何手动调整。此外，它还能够估计和监测多个幼虫在不同程度的疼痛刺激下的身体活动。所开发的试验台的性能是用不同的刺激水平对许多测试批次的斑马鱼幼虫进行实验评估。所获得的结果表明，准确跟踪和监测多种幼虫对不同水平的疼痛刺激的反应。

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2015 IEEE Jordan Conference on Applied Electrical Engineering and Computing Technologies (AEECT)

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