Epitope study and cDNA screening of major surface glycoprotein of Pneumocystis carinii.

The Journal of protozoology Pub Date : 1991-11-01
Y Nakamura, K Kitada, M Wada, M Saito
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Abstract

Use of monoclonal antibodies against the major glycoprotein of Pneumocystis carinii (P115) implicated the sugar moiety as being strongly antigenic. Furthermore, monoclonal antibodies directed against the peptide portion of P115 were generated by using synthetic oligopeptides after amino acid sequencing was done on P115 proteolytic fragments.

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卡氏肺囊虫主要表面糖蛋白的表位研究及cDNA筛选。
使用针对卡氏肺囊虫(P115)主要糖蛋白的单克隆抗体表明糖部分具有强抗原性。此外,对P115蛋白水解片段进行氨基酸测序后,利用合成的寡肽制备了针对P115肽段的单克隆抗体。
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Proliferation patterns of latent Pneumocystis carinii in rat organs during progressive stages of immunosuppression. 7th European Conference on Cell and Molecular Biology of Ciliates. Toledo, Spain, September 2-6, 1991. Abstracts. The Society of Protozoologists. 1991 Abstracts. John O. Corliss--ciliatologist extraordinaire. Calcium transport and compartment analysis of free and exchangeable calcium in Plasmodium falciparum-infected red blood cells.
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