Genetic engineering of stem cells by non-viral vectors

1st Portuguese Biomedical Engineering Meeting Pub Date : 2011-03-01 DOI:10.1109/ENBENG.2011.6026044

C. Madeira, S. Ribeiro, R. Mendes, Irina Pinheiro, C. L. da Silva, J. Cabral

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引用次数: 1

Abstract

Stem/progenitor cells hold a great promise for application in several therapies due to their unique biological characteristics. With the purpose of harnessing these cells full potential in cell-or gene-based therapies it might be advantageous to enhance some of their features through gene delivery strategies. Accordingly, we are interested in developing efficient and safe methodologies to genetically engineer stem cells, boosting their therapeutic efficacy in Regenerative Medicine. In our work, delivery of plasmid DNA to human Bone Marrow Mesenchymal Stem Cells (BM-MSC) was optimized by lipofection and by a recently available microporation technique and no effect was observed in their immunophenotypic characteristics or differentiative potential. After lipofection similar number of plasmid copies was determined at different cell passages. Importantly, cell proliferation kinetics slowed down due to the presence of plasmid. Overall, we believe our findings are extremely useful towards the maximization of gene delivery to human MSC, without compromising cell function and viability.

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非病毒载体干细胞的基因工程

干细胞/祖细胞由于其独特的生物学特性，在多种治疗中具有很大的应用前景。为了在细胞或基因治疗中充分利用这些细胞的潜力，通过基因传递策略增强它们的某些特征可能是有利的。因此，我们有兴趣开发高效和安全的方法来基因工程干细胞，提高其在再生医学中的治疗效果。在我们的工作中，质粒DNA递送到人骨髓间充质干细胞(BM-MSC)通过脂肪转染和最近可用的微孔技术进行优化，并没有观察到其免疫表型特征或分化潜力的影响。脂质转染后，在不同的细胞传代中确定了相似的质粒拷贝数。重要的是，由于质粒的存在，细胞增殖动力学减慢。总的来说，我们相信我们的发现对于在不影响细胞功能和活力的情况下最大限度地将基因传递到人间充质干细胞非常有用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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1st Portuguese Biomedical Engineering Meeting

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