Effect of Nano-HAP on Proliferation of Hepatocellular Carcinoma Cells

Abstract

Recently, the treatments of hepatocellular carcinoma with nano-hydroxyapatite (nano-HAP) particles have been given much attention. In this study, a new kind of nano-HAP particles was prepared by a homogenous method, and these particle diameters and their distribution, crystallization degree, chemical bondings, and morphology were characterized by laser scattering particle analyzer, X-ray diffraction, FTIR spectrum, transmission electron microscope, and electron diffraction. The results showed that the average particle diameter of nano-HAP was 59.9 nm, the prepared nano-HAP was amorphous and having a low crystallization degree. Furthermore, the characteristic absorption peaks of HAP appeared in FTIR spectra and the electron diffraction annuli was also clear. The prepared nano-HAP particles were used for the treatment of hepatocellular carcinoma. The inhibition effect was determined in vitro for three different concentrations by MTT methods. The results demonstrated that the prepared nano-HAP with a size of 59.9 nm most effectively inhibited the proliferation of hepatocellular carcinoma cells and the inhibition rate was more than 70%.