Methodological approaches regarding bacteriological diagnostics of actinobacillus pleuropneumonia infection in pigs

Y. Y. Ayshpur, I. Mushtuk, V. V. Gumenyuk, O. M. Ermolenko, M. M. Derevianko
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Abstract

The culture was determined as pathogenic in cases of death of at least 2 white mice within 24-72 hours, followed by bacteriological examination of the pathological material and subsequent isolation of the culture of the pathogen. As an additional study disks with saponin – 750 μg/disk, bacitracin – 10 μg/disk, erythromycin – 15 μg/disk, azithromycin – 15 μg/disk were used to identify actinobacillus. Conclusions and prospects for further research. As a result of the conducted researches the sequence of research is described, methodical instructions concerning bacteriological diagnosis of APP of pigs are developed and improved, the scheme of researches is presented, cultures of Actinobacillus pleuropneumoniae from pathological material are isolated. Given that we have proven the prevalence of APP in pigs in domestic farms, bacteriological examination for the presence of its pathogen remains relevant.
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猪胸膜肺炎放线杆菌感染细菌学诊断方法探讨
在24-72小时内至少有2只小白鼠死亡的情况下,确定培养物具有致病性,然后对病理材料进行细菌学检查,随后分离培养物的病原体。另外用皂苷- 750 μg/片、杆菌素- 10 μg/片、红霉素- 15 μg/片、阿奇霉素- 15 μg/片检测放线菌。结论及进一步研究的展望。根据所进行的研究,描述了研究的顺序,制定和改进了猪APP细菌学诊断的系统说明,提出了研究方案,从病理材料中分离出胸膜肺炎放线杆菌培养物。鉴于我们已经证明了家庭农场猪中APP的流行,因此对其病原体进行细菌学检查仍然是相关的。
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