[Basaliom. Case report and isolation of mesenchymal stroma cells in various growth conditions in vitro].

Abstract

The case of a 54-year-old man with multiple basaliomas of the face but without any other symptoms of the Goltz-Gorlin syndrome is described. Obviously, there ist a family history of a tendency to develop basaliomas. One of the patient's three brothers, his mother, her sister and two of his six children were also affected. After removal of the tumor, electron microscopy revealed intranuclear crystal-like structures in some of the epithelial cells of the tumor tissue. The particular geometry of these structures suggests that these are papilloma viruses. The tumor stroma contained fibroblasts, fibrocytes and predominantly myofibroblasts. Tumor explants were cultured in vitro in Dulbecco-Vogt medium using 5 different supplements: 10% fetal calf serum (FCS), 10% fresh human serum with and without PDGF and CL (PDGF-CL, PDGF, CL, NULL). These cultures result in different rates of collagen gel contraction and binding of varying amounts of the "q" fraction of the first complement (C1q): PDGF CL-cells greater than PDGF cells greater than FCS cells greater than NULL cells. The culture of tumor tissue cells in different media thus seems to enhance the dominance of different cell subtypes.