Comparative Study on the Extraction and Quantification of Polyphenols from Moringa oleifera Leaves Using Maceration with Pulsed Electric Field (PEF) as Pretreatment and Microwave-Assisted Extraction (MAE)

Sukardi, Puput Safitri, H. Setyawan
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Abstract

Moringa oleifera leaves are rich in nutrients and bioactive compounds such as polyphenols which can be used as an antioxidant. Polyphenols of Moringa leaves can be extracted using conventional and non-conventional methods. This study compared maceration with Pulsed Electric Field (PEF) pretreatment and MAE method on extracting polyphenols from Moringa leaves. The performance of those methods was assessed based on the yield extracts, total polyphenol content (TPC), and antioxidant activity (IC50). The results showed that different methods significantly affected TPC and antioxidant activity. Maceration with PEF pretreatments obtained extracts with the highest TPC value (179.11 mg GAE/g) and antioxidant activity (33.56 ppm). In comparison, the MAE method produced TPC and antioxidant activity values of 22.64 mg GAE/g and 54.59 ppm, respectively. The yield extracts did not show differences significantly. Maceration with PEF pretreatment and MAE obtained the yield extract of 12.62% and 13.16%, respectively. Based on the results, it can be seen that maceration with PEF pretreatment has a better ability to increase TPC and antioxidant activity compared to the MAE method. Maceration with PEF pretreatment can increase TPC up to 7.9 times and antioxidant activity up to 1.6 times. This study showed the potential of PEF pretreatment in increasing yield and recovery of total polyphenol content and antioxidant activity of Moringa leaves extracts. Further study needs to be done focusing on optimization of extraction using maceration with PEF pretreatment characteristics, such as electric field strength, frequency, and exposure time.
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脉冲电场浸渍法(PEF)和微波辅助提取法(MAE)提取辣木叶中多酚的比较研究
辣木叶含有丰富的营养和生物活性化合物,如多酚,可以用作抗氧化剂。辣木叶中的多酚可采用常规和非常规方法提取。比较了浸出法、脉冲电场预处理法和MAE法对辣木叶中多酚的提取效果。根据提取物的产量、总多酚含量(TPC)和抗氧化活性(IC50)对这些方法的性能进行了评价。结果表明,不同处理方法对TPC和抗氧化活性有显著影响。PEF预处理浸出的提取物TPC值最高(179.11 mg GAE/g),抗氧化活性最高(33.56 ppm)。相比之下,MAE法得到的TPC和抗氧化活性值分别为22.64 mg GAE/g和54.59 ppm。提取物的产量差异不显著。PEF预处理浸出率为12.62%,MAE浸出率为13.16%。综上所述,PEF预处理浸渍比MAE法具有更好的提高TPC和抗氧化活性的能力。PEF预处理浸渍可使TPC提高7.9倍,抗氧化活性提高1.6倍。本研究表明,PEF预处理在提高辣木叶提取物的收率和总多酚含量的回收率以及抗氧化活性方面具有较大的潜力。PEF预处理的电场强度、频率、暴露时间等参数对浸渍提取工艺的影响有待进一步研究。
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