[Normal and experimental microcirculation in the liver (study of corrosion preparations using scanning electron microscopy)].

Abstract

Liver vascular bed of rat is reported to be studied by scanning electron microscopy of corrosive sections. These were prepared with commercially produced methylmetacrylate monomer which was prepolymerized and injected into the prewashed and fixed vascular bed. The injections have been provided through the left cardial ventricle or directly into the liver vessels (hepatic artery, portal vein or hepatis veines). Samples from injected liver were preleved with subsequent corrosion in 40% KOH. The obtained corrosion casts were sectioned using the operational microscope and modified to be observed by scanning electron microscope Tesla BS 300. Normal liver rat pattern has been studied by scanning electron microscopy on the level of angioarchitectonics of vascular bed. The interrelation between branches of hepatic artery and portal vein was documented including their connection with evacuative venous system. In the second part of work the same method was used to study changes of configuration of liver vascular bed in rat through the early and later phases of liver regeneration after partial hepatectomy.