{"title":"Activity of phenacetin O-deethylase in rat liver: effect of inducers and inhibitors in vivo.","authors":"A Starek","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A method for the determination of microsomal phenacetin O-deethylase activity in the rat liver was described. Optimum conditions in which phenacetin is enzymatically converted to N-acetyl-p-aminophenol were determined. The product of enzymatic reaction was acid-hydrolyzed while heating. The amount of p-aminophenol formed was determined by means of indophenol reaction. The described method was applied to assess activity of phenacetin O-deethylase in normal rats and rats treated with inducers or inhibitors of microsomal monooxygenases, phenobarbital, 3,4-benzpyrene, SKF 525-A and cobaltous chloride, respectively.</p>","PeriodicalId":20359,"journal":{"name":"Polish journal of occupational medicine and environmental health","volume":"4 1","pages":"67-75"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polish journal of occupational medicine and environmental health","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
A method for the determination of microsomal phenacetin O-deethylase activity in the rat liver was described. Optimum conditions in which phenacetin is enzymatically converted to N-acetyl-p-aminophenol were determined. The product of enzymatic reaction was acid-hydrolyzed while heating. The amount of p-aminophenol formed was determined by means of indophenol reaction. The described method was applied to assess activity of phenacetin O-deethylase in normal rats and rats treated with inducers or inhibitors of microsomal monooxygenases, phenobarbital, 3,4-benzpyrene, SKF 525-A and cobaltous chloride, respectively.
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