[The cytoskeleton of cultured spinal cord cells from mouse embryos].

Abstract

By means of monoclonal antibodies (fluorescein-isothiocyanate- and rhodamine-labelled) distribution and quantitative content of the main cytoskeleton proteins (actin, tubulin, neurofilamentous protein with the molecular mass of 160 kDa and glial fibrillar acid protein) has been studied in various types of the mouse embryos spinal cord cells, cultivated in monolayer. During the process of development of neurons tubulin displaces from the neuronal soma into its processes with its predominant concentration in some of them, which are probably more active functionally at certain stages of differentiation. The total amount of tubulin is supposed to remain stable during the neuron life time. Quantitative content and distribution of actin filaments in various types of the cells are different. Actin content in the neurons is much lower than in glial cells and fibroblasts. The major amount of protein (neurofilamentous, glial fibrillar acid protein) is concentrated in cell bodies and in proximal parts of the processes. The pattern of distribution of the cytoskeleton proteins in the spinal cord cells has been revealed.