Activation of muscarinic and serotonergic receptors results in phosphoinositide hydrolysis but not in mobilization of calcium in sympathetic neurons.

Blood vessels Pub Date : 1991-01-01 DOI:10.1159/000158837
T D Wakade, A S Bhave, S V Bhave, A R Wakade
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引用次数: 3

Abstract

The effects of various neurotransmitters on phosphoinositide hydrolysis, mobilization of Ca2+ and release of [3H]-norepinephrine ([3H]-NE) were studied in cultures of sympathetic neurons of chick embryos. [3H]-inositol-1,4,5-triphosphate ([3H]-IP3) was increased in sympathetic neurons by acetylcholine (ACh), muscarine and serotonin (5-HT). Dopamine and norepinephrine did not stimulate phosphoinositide hydrolysis. Intracellular concentration of free Ca2+ ([Ca2+]i) was measured in Indo-1-loaded sympathetic neurons at rest and after addition of test agents. Measurements were made in the cell body and growth cone regions since Ca2+ mobilization is known to be different in different regions of the sympathetic neurons. ACh (nicotinic component was blocked by hexamethonium) and 5-HT failed to increase the [Ca2+]i, in the cell body as well as in the growth cone. The spontaneous release of [3H]-NE was not affected by ACh and 5-HT. Caffeine increased the [Ca2+]i only in the cell body but not in the growth cone and had no effect on the release of [3H]-NE. These results suggest that an IP3-insensitive but caffeine-sensitive pool of Ca2+ is present only in the somatic region of sympathetic neurons and is not coupled to the transmitter release.

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毒菌碱和血清素能受体的激活导致磷酸肌肽水解,但不导致交感神经元中钙的动员。
研究了不同神经递质对鸡胚交感神经元磷酸化肌肽水解、Ca2+动员和[3H]-去甲肾上腺素([3H]-NE)释放的影响。[3H]-肌醇-1,4,5-三磷酸([3H]-IP3)在乙酰胆碱(ACh)、肌碱和血清素(5-HT)作用下升高。多巴胺和去甲肾上腺素不刺激磷酸肌肽水解。在静止状态和添加试验剂后,测量了携带印-1的交感神经元细胞内游离Ca2+ ([Ca2+]i)的浓度。由于已知交感神经元的不同区域Ca2+动员不同,因此在细胞体和生长锥区域进行了测量。ACh(烟碱成分被六甲铵阻断)和5-HT未能增加细胞体和生长锥内的[Ca2+]i。[3H]-NE的自发释放不受ACh和5-HT的影响。咖啡因只增加了细胞体内的[Ca2+]i,而没有增加生长锥内的[3H]-NE的释放。这些结果表明,ip3不敏感但咖啡因敏感的Ca2+池仅存在于交感神经元的体细胞区域,不耦合于递质释放。
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