Activation of muscarinic and serotonergic receptors results in phosphoinositide hydrolysis but not in mobilization of calcium in sympathetic neurons.

Blood vessels Pub Date : 1991-01-01 DOI:10.1159/000158837
T D Wakade, A S Bhave, S V Bhave, A R Wakade
{"title":"Activation of muscarinic and serotonergic receptors results in phosphoinositide hydrolysis but not in mobilization of calcium in sympathetic neurons.","authors":"T D Wakade,&nbsp;A S Bhave,&nbsp;S V Bhave,&nbsp;A R Wakade","doi":"10.1159/000158837","DOIUrl":null,"url":null,"abstract":"<p><p>The effects of various neurotransmitters on phosphoinositide hydrolysis, mobilization of Ca2+ and release of [3H]-norepinephrine ([3H]-NE) were studied in cultures of sympathetic neurons of chick embryos. [3H]-inositol-1,4,5-triphosphate ([3H]-IP3) was increased in sympathetic neurons by acetylcholine (ACh), muscarine and serotonin (5-HT). Dopamine and norepinephrine did not stimulate phosphoinositide hydrolysis. Intracellular concentration of free Ca2+ ([Ca2+]i) was measured in Indo-1-loaded sympathetic neurons at rest and after addition of test agents. Measurements were made in the cell body and growth cone regions since Ca2+ mobilization is known to be different in different regions of the sympathetic neurons. ACh (nicotinic component was blocked by hexamethonium) and 5-HT failed to increase the [Ca2+]i, in the cell body as well as in the growth cone. The spontaneous release of [3H]-NE was not affected by ACh and 5-HT. Caffeine increased the [Ca2+]i only in the cell body but not in the growth cone and had no effect on the release of [3H]-NE. These results suggest that an IP3-insensitive but caffeine-sensitive pool of Ca2+ is present only in the somatic region of sympathetic neurons and is not coupled to the transmitter release.</p>","PeriodicalId":9009,"journal":{"name":"Blood vessels","volume":"28 1-3","pages":"6-10"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000158837","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Blood vessels","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1159/000158837","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3

Abstract

The effects of various neurotransmitters on phosphoinositide hydrolysis, mobilization of Ca2+ and release of [3H]-norepinephrine ([3H]-NE) were studied in cultures of sympathetic neurons of chick embryos. [3H]-inositol-1,4,5-triphosphate ([3H]-IP3) was increased in sympathetic neurons by acetylcholine (ACh), muscarine and serotonin (5-HT). Dopamine and norepinephrine did not stimulate phosphoinositide hydrolysis. Intracellular concentration of free Ca2+ ([Ca2+]i) was measured in Indo-1-loaded sympathetic neurons at rest and after addition of test agents. Measurements were made in the cell body and growth cone regions since Ca2+ mobilization is known to be different in different regions of the sympathetic neurons. ACh (nicotinic component was blocked by hexamethonium) and 5-HT failed to increase the [Ca2+]i, in the cell body as well as in the growth cone. The spontaneous release of [3H]-NE was not affected by ACh and 5-HT. Caffeine increased the [Ca2+]i only in the cell body but not in the growth cone and had no effect on the release of [3H]-NE. These results suggest that an IP3-insensitive but caffeine-sensitive pool of Ca2+ is present only in the somatic region of sympathetic neurons and is not coupled to the transmitter release.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
毒菌碱和血清素能受体的激活导致磷酸肌肽水解,但不导致交感神经元中钙的动员。
研究了不同神经递质对鸡胚交感神经元磷酸化肌肽水解、Ca2+动员和[3H]-去甲肾上腺素([3H]-NE)释放的影响。[3H]-肌醇-1,4,5-三磷酸([3H]-IP3)在乙酰胆碱(ACh)、肌碱和血清素(5-HT)作用下升高。多巴胺和去甲肾上腺素不刺激磷酸肌肽水解。在静止状态和添加试验剂后,测量了携带印-1的交感神经元细胞内游离Ca2+ ([Ca2+]i)的浓度。由于已知交感神经元的不同区域Ca2+动员不同,因此在细胞体和生长锥区域进行了测量。ACh(烟碱成分被六甲铵阻断)和5-HT未能增加细胞体和生长锥内的[Ca2+]i。[3H]-NE的自发释放不受ACh和5-HT的影响。咖啡因只增加了细胞体内的[Ca2+]i,而没有增加生长锥内的[3H]-NE的释放。这些结果表明,ip3不敏感但咖啡因敏感的Ca2+池仅存在于交感神经元的体细胞区域,不耦合于递质释放。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Contractile and morphologic properties of a saphenous vein after 12 years as an aortocoronary bypass graft. Effect of H-8, an isoquinolinesulfonamide inhibitor of cyclic nucleotide-dependent protein kinase, on cAMP- and cGMP-mediated vasorelaxation. Hemorheological effects of buflomedil: action on shape and functions of the human neutrophils. Norepinephrine, phentolamine and buflomedil influence on arteriolar vasomotion in the hamster skinfold preparation. Heme-dependent activation of guanylate cyclase by nitric oxide: a novel signal transduction mechanism.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1