Effect of inhaled N-acetylcysteine on the oxidative stress and content of proteases in lungs of newborn guinea pigs exposed to hyperoxia

Lung and airway developmental biology Pub Date : 2018-09-15 DOI:10.1183/13993003.CONGRESS-2018.PA1387

I. Katovich, Zhanna Rutkouskaya, S. Anischenko, A. Tahanovich

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Abstract

Lung-protective pharmacotherapies preventing development of bronchopulmonary dysplasia (BPD) in infants are widely investigated. The aim of this study was to evaluate the influence of inhaled N-acetylcysteine (NAC) on the oxidative and protease imbalance in lungs of newborn guinea pigs under prolonged hyperoxia. The studied groups included: “hyperoxia” (n=12, 70% O2, 14 days), “hyperoxia+NAC” (n=6, 250 mg/kg of NAC introduced by nebulizer on alternate days on hyperoxia exposure), “control” (n=12, room air). Hyperoxia exposure resulted in enhanced production of reactive oxygen species (ROS) by bronchoalveolar lavage cells, 1,9-fold increased level of products reacting with thiobarbituric acid (TBARP), low glutathione peroxidase activity and glutathione (G-SH) in bronchoalveolar lavage fluid (BALF) (30% and 68% of controls respectively); elevated alpha1-antitrypsin (A1-AT), neutrophil elastase and matrix metalloproteases MMP2 and MMP9 in lung homogenates (141%, 286%, 132%, and 176% of controls respectively) (for all p

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吸入n -乙酰半胱氨酸对高氧新生豚鼠肺氧化应激及蛋白酶含量的影响

预防婴儿支气管肺发育不良(BPD)发展的肺保护药物治疗被广泛研究。本研究旨在探讨长期高氧条件下吸入n -乙酰半胱氨酸(NAC)对新生豚鼠肺部氧化和蛋白酶失衡的影响。研究组包括:“高氧组”(n=12, 70% O2, 14天)、“高氧+NAC组”(n=6，在高氧暴露时隔天使用雾化器向NAC注入250 mg/kg)、“对照组”(n=12，室内空气)。高氧暴露导致支气管肺泡灌洗液细胞活性氧(ROS)产生增加，与硫代巴比妥酸(TBARP)、低谷胱甘肽过氧化物酶活性和谷胱甘肽(G-SH)反应的产物水平增加1.9倍(分别为对照组的30%和68%);肺匀浆中α - 1抗胰蛋白酶(A1-AT)、中性粒细胞弹性蛋白酶和基质金属蛋白酶MMP2和MMP9升高(分别为对照组的141%、286%、132%和176%)

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Lung and airway developmental biology

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