A novel electrochemical biosensor for bone turnover detection based on molecular imprinting technology

Abstract

This research reports a novel label-free sensing method for the measurement of C-telopeptide of Type-I collagen (CTX-1) in order to monitor bone turnover at an early stage. An interdigital sensor jointly with Electrochemical Impedance Spectroscopy (EIS) was used to study the resistive and capacitive properties of samples. Molecular Imprinted Polymers (MIPs) containing recognition sites for CTX-1 molecules were synthesized. The sensor was coated with the prepared MIPs to increase the sensitivity of the sensor to CTX-1. Different known concentration samples were tested and the sensitivity curve was plotted. The Nyquist fitted Curve and its equivalent Randle's circuit was estimated using a complex non-linear least square method (CNLS). The initial results are promising for further development of an inexpensive and portable device for early detection of bone turnover, so therapy can be started earlier.