Bakteri Endofit F4 dari Daun Pepaya (Carica papaya L): Potensinya sebagai Penghasil Enzim Ekstraseluler

Purbowatiningrum Ria Sarjono, Ismiyarto Ismiyarto, Ngadiwiyana Ngadiwiyana, Norita Prasetya
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Abstract

AbstractPapaya leaves are known to have antibacterial activity, so the endophytic bacteria on the papaya leaves are thought to have antibacterial activity. Previous studies have obtained 5 isolates of endophytic bacteria from papaya leaves, including F1, F2, F3, F4 and F5. Endophytic bacteria F1, F3, and F5 have known antibacterial activity, while endophytic bacteria F4 have not yet explored their activity in producing extracellular enzymes, so that in this study a qualitative test of extracellular enzymes was carried out on endophytic bacteria F4. The purpose of this study was to obtain confirmation of morphology, qualitative phytochemical data, and the ability to produce extracellular enzymes lipase, cellulase, protease and amylase from endophytic bacteria F4.The results obtained were F4 bacterial isolates white, round and smooth rounded edges which were gram positive with single cell morphology and the shape is bacillus (stems). F4 bacteria have the potential to contain amylase and protease extracellular enzymes.
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木瓜叶的内生细菌F4:它能产生细胞外酶
摘要已知木瓜叶具有抗菌活性,因此认为木瓜叶上的内生细菌具有抗菌活性。前期研究从木瓜叶片中分离得到5株内生细菌,分别为F1、F2、F3、F4和F5。内生细菌F1、F3、F5具有已知的抑菌活性,而内生细菌F4尚未对其胞外酶的产生活性进行探索,因此本研究对内生细菌F4进行胞外酶的定性检测。本研究的目的是确认内生细菌F4的形态、定性植物化学数据以及产生胞外酶脂肪酶、纤维素酶、蛋白酶和淀粉酶的能力。结果F4菌株分离物为白色、圆形、光滑的圆形边缘,革兰阳性,呈单细胞形态,形状为芽孢杆菌(茎)。F4细菌可能含有淀粉酶和蛋白酶胞外酶。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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