{"title":"L-arginine does not restore endothelial dysfunction in atherosclerotic rabbit aorta in vitro.","authors":"A Mügge, D G Harrison","doi":"10.1159/000158881","DOIUrl":null,"url":null,"abstract":"<p><p>Bioassay studies suggest that impaired endothelium-dependent relaxation in atherosclerotic arteries is due to a reduced release of biologically active endothelium-derived relaxing factor (EDRF). We tested the hypothesis that endothelial dysfunction is caused by deficiency of the EDRF precursor L-arginine. Aortae from normal and cholesterol-fed (1%, 4 months) rabbits were excised and incubated for 1 h with 5 mM L-arginine. Pretreatment with L-arginine had no effect on the relaxation to acetylcholine in normal vessels and was without effect on the impaired response of atherosclerotic arteries to acetylcholine. This finding suggests that L-arginine deficiency is unlikely the underlying cause of impaired endothelium-dependent relaxation in the aorta of cholesterol-fed rabbits.</p>","PeriodicalId":9009,"journal":{"name":"Blood vessels","volume":"28 5","pages":"354-7"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000158881","citationCount":"55","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Blood vessels","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1159/000158881","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 55
Abstract
Bioassay studies suggest that impaired endothelium-dependent relaxation in atherosclerotic arteries is due to a reduced release of biologically active endothelium-derived relaxing factor (EDRF). We tested the hypothesis that endothelial dysfunction is caused by deficiency of the EDRF precursor L-arginine. Aortae from normal and cholesterol-fed (1%, 4 months) rabbits were excised and incubated for 1 h with 5 mM L-arginine. Pretreatment with L-arginine had no effect on the relaxation to acetylcholine in normal vessels and was without effect on the impaired response of atherosclerotic arteries to acetylcholine. This finding suggests that L-arginine deficiency is unlikely the underlying cause of impaired endothelium-dependent relaxation in the aorta of cholesterol-fed rabbits.
生物测定研究表明,粥样硬化动脉中内皮依赖性松弛受损是由于生物活性内皮源性松弛因子(EDRF)的释放减少。我们检验了内皮功能障碍是由EDRF前体l -精氨酸缺乏引起的假设。取正常和4月龄(1%)高胆固醇家兔主动脉,用5 mM l-精氨酸孵育1小时。l -精氨酸预处理对正常血管对乙酰胆碱的松弛无影响,对动脉粥样硬化对乙酰胆碱的反应受损无影响。这一发现表明,l-精氨酸缺乏不太可能是胆固醇喂养家兔主动脉内皮依赖性松弛受损的根本原因。