Growth responses in isolated elastic, muscular and resistance-sized arterial segments of the rat.

Blood vessels Pub Date : 1991-01-01 DOI:10.1159/000158884
J G De Mey, M P Uitendaal, H C Boonen, P M Schiffers, G E Fazzi
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引用次数: 23

Abstract

To evaluate whether intravascular phenomena contribute to local differences in growth responses of the arterial wall, we evaluated responses to organoid culture in a broad variety of arterial preparations. Arterial segments were isolated from adult, normotensive rats, sympathectomized, denuded from endothelium, and suspended in medium supplemented with serum. As judged from the nuclear incorporation of the thymidine analogue 5-bromo-2'-deoxyuridine (BrdUrd), this induced a transient stimulation of DNA synthesis in only a fraction of the arterial smooth muscle cells in all types of arteries. This intramedial DNA synthesis was more marked in renal arteries than in carotid arteries or aortae and was least pronounced in main pulmonary, femoral, and superior mesenteric artery and in mesenteric resistance-sized arteries. Organoid culture of isolated arteries did not increase the cross-sectional area of the media or the number of medial cells. It rather resulted in proliferation of smooth-muscle-like cells outside the media. In addition, smooth-muscle-like cells migrated out of the isolated arterial segments during culture. The rate of proliferation of these isolated cells did not differ between large arteries of different anatomical origin. However, isolated cells derived from mesenteric resistance arteries proliferated at a rate that was 4 times slower than that of large artery cells. The presence of endothelium significantly reduced medial DNA synthesis in carotid and renal artery segments, but not in mesenteric resistance-sized preparations. These data indicate that growth responses of the arterial wall differ quantitatively with the anatomical location and branching order of the vascular segment. In addition to the regional heterogeneity of endothelial effects on mitogenic responses of arterial smooth muscle, this seems to be due to regional differences in the susceptibility of arterial smooth muscle to exogenous growth factors. In this respect, we speculate that subsets of growth-resistant and growth-prone arterial smooth muscle cells could be heterogeneously distributed over the arterial tree.

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大鼠离体动脉弹性、肌肉和阻力段的生长反应。
为了评估血管内现象是否会导致动脉壁生长反应的局部差异,我们评估了各种动脉制剂对类器官培养的反应。从正常血压的成年大鼠身上分离动脉段,切除交感神经,剥去内皮,悬浮在添加血清的培养基中。从胸腺嘧啶类似物5-溴-2'-脱氧尿嘧啶(BrdUrd)的核结合判断,这只在所有类型动脉的一小部分动脉平滑肌细胞中诱导了DNA合成的短暂刺激。这种内侧DNA合成在肾动脉中比在颈动脉或主动脉中更为明显,而在肺主动脉、股动脉、肠系膜上动脉和肠系膜阻力动脉中最不明显。离体动脉的类器官培养没有增加培养基的横截面积或中间细胞的数量。相反,它导致了介质外平滑肌样细胞的增殖。此外,在培养过程中,平滑肌样细胞从分离的动脉段中迁移出来。这些分离细胞的增殖速度在不同解剖来源的大动脉之间没有差异。然而,来自肠系膜抵抗动脉的分离细胞的增殖速度比大动脉细胞慢4倍。内皮的存在显著降低了颈动脉和肾动脉段内侧DNA的合成,但在肠系膜阻力大小的制剂中没有。这些数据表明,动脉壁的生长反应在数量上随血管段的解剖位置和分支顺序而不同。除了内皮对动脉平滑肌有丝分裂反应的影响存在区域异质性外,这似乎是由于动脉平滑肌对外源性生长因子的易感性存在区域差异。在这方面,我们推测生长抵抗和易生长的动脉平滑肌细胞亚群可能在动脉树中分布不均。
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