Effects of Nanosecond Pulsed Electric Fields Application and Combination of Anticancer Drug on Cancer Cell

2019 IEEE Pulsed Power & Plasma Science (PPPS) Pub Date : 2019-06-01 DOI:10.1109/PPPS34859.2019.9009682

S. Enomoto, Y. Yamamoto, D. Konishi, M. Futawaka, Y. Kusuhashi, K. Teranishi, Y. Uto, N. Shimomura

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引用次数: 1

Abstract

In recently years, malignant neoplasm (cancer) occupies the extremely high percentage of cause of death, there are disadvantages to current cancer therapy. It has been confirmed nanosecond pulsed electric fields (nsPEFs) application induced apoptosis on cancer cells; many studies applying it to a cancer therapy have been started. We applied nsPEFs to mouse melanoma cells: B16-F10 in a cuvette. In addition, we combine nsPEFs application and anticancer drug (Adriamycin) administration, and cancer surviving fraction was measured by Crystal Violet Assay. The nsPEFs application or anticancer drug (Adriamycin) administration significantly decreased the cell surviving fraction. The cell survival fraction by combinational treatment of nsPEFs application and Adriamycin administration was significantly smaller than the unilateral treatment. Apoptosis ratio was higher in the combinational treatment. NsPEFs application might make nanoscale holes in the cell membrane, and the anticancer drug could work more effective.

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纳秒脉冲电场应用及联合抗癌药物对癌细胞的影响

近年来，恶性肿瘤(癌症)在死亡原因中所占比例极高，目前的癌症治疗存在诸多弊端。应用纳秒脉冲电场(nsPEFs)诱导肿瘤细胞凋亡已被证实;许多将其应用于癌症治疗的研究已经开始。我们将nsPEFs应用于小鼠黑色素瘤细胞:B16-F10。此外，我们将nsPEFs与抗癌药物(阿霉素)联合应用，并采用结晶紫法测定肿瘤存活分数。应用nsPEFs或使用抗癌药物(阿霉素)可显著降低细胞存活率。应用nsPEFs和阿霉素联合治疗的细胞存活率明显小于单方治疗。联合治疗组细胞凋亡率较高。NsPEFs的应用可能会在细胞膜上制造纳米级的孔，而抗癌药物可能会更有效。

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2019 IEEE Pulsed Power & Plasma Science (PPPS)

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