{"title":"[Immunobiological characteristics of Mycoplasma hyopneumoniae antigens and the diagnosis of infection caused by this organism].","authors":"A Ia Pustovar","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The paper presents the developed variants of enzyme immunoassay (EIA) using two M. hyopneumoniae (R-14 strain) antigens obtained by biomass freezing and thawing and two Tween-dissolved antigens by gel chromatography. To identify the cultures isolated, an indirect immunofluorescence reaction was proposed by employing agar blocks. The developed methods made it possible to identify 23 bovine-isolated cultures as M. hyopneumoniae antigens. The latter were detected by EIA in diagnostically significant titers in serum and colostrum samples from 2 swine-breeding farms. A negative result was obtained in the respiratory mycoplasmosis-free farms.</p>","PeriodicalId":76796,"journal":{"name":"Vestnik Akademii meditsinskikh nauk SSSR","volume":" 6","pages":"55-7"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vestnik Akademii meditsinskikh nauk SSSR","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The paper presents the developed variants of enzyme immunoassay (EIA) using two M. hyopneumoniae (R-14 strain) antigens obtained by biomass freezing and thawing and two Tween-dissolved antigens by gel chromatography. To identify the cultures isolated, an indirect immunofluorescence reaction was proposed by employing agar blocks. The developed methods made it possible to identify 23 bovine-isolated cultures as M. hyopneumoniae antigens. The latter were detected by EIA in diagnostically significant titers in serum and colostrum samples from 2 swine-breeding farms. A negative result was obtained in the respiratory mycoplasmosis-free farms.
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