Gene polymorphisms and risk of preeclampsia in Egyptian women

Abstract

Aim: The aim of this study was to evaluate the possible association between ACE I/D, AT1 receptor 1166 A:C, AT2receptor-1332 A:G, and MMP-9-1562 C:T polymorphisms and risk of preeclampsia in Egyptian women.Materials and Methods: This case-control study included 108 pregnant women was allocated into two groups, 54pre-eclamptic women group and control group which included 54 normotensive pregnant women. Genotyping of AT1 1166A:C and AT2 −1332 A: G were performed by duplex polymerase chain reaction-restriction fragment length polymorphismPCR-RFLP. Genotyping of I/D polymorphism of ACE was carried out by PCR and genotyping of MMP-9 −1562C/T wasperformed by tetra-primer amplification refractory mutation system T- ARMS–PCR.Results: The DD genotype of ACE gene was significantly associated with increased risk of preeclampsia[OR (95% CI) = 2.47 (0.72–8.5), p = 0.02] and the D allele was significantly associated with an increased risk ofpreeclampsia [OR (95% CI) = 1.95 (1.08–3.54), p = 0.02]. The AT2 GG genotype frequency was significantly higher inpreeclampsia [OR (95% CI) = 3.24 (1.25–8.41), p = 0.002] and the G allele [OR (95% CI) = 2.41 (1.39–4.18), p = 0.002].  However, the AT1 CC and MMP9 TT genotypes frequency were insignificantly associated with preeclampsia.Conclusion: ACE gene I/D and -1332A/G of AT2 receptor polymorphisms, but not AT1 receptor gene A1166C andMMP-9 (-1562 C/T) polymorphisms, could be related to the risk of preeclampsia in Egyptian women.