Production of Pure Aflatoxin B1 (AFB1) Using Different Media

Abstract

Aflatoxin B1 (AFB1) is a mycotoxin that is produced by Aspergillus flavus as a secondary metabolite. This study aimed to select the optimal medium to grow A. flavus to produce the highest concentration of AFB1. Solid-state fermentation of rice, corn, and a mixture of corn, sugar, peanut, and coconut (CSPC), and semisynthetic liquid culture of Yeast Extract sucrose Broth (YEB), Yeast Extract Sucrose Broth with Peptone (YEBP), and Czapek-Dox liquid Medium (Czp-D) were tested in this study. The AFB1 was extracted using the organic solvents method and purified through Thin Layer Chromatography (TLC). The concentration of AFB1 was then estimated using High-Performance Liquid Chromatography (HPLC). The results showed that the Czp-D medium had the potential to produce the highest concentration of AFB1, while the lowest concentration of AFB1 was gained when using the rice medium.