Nutritional content and microbial contamination of fresh cold and frozen Bali beef in Mambal RPH production in Badung Regency, Bali Province

Abstract

This study aims to determine the nutrition and microbial contamination of fresh, chilled, and frozen Bali beef. This study used a completely randomized design (CRD) direct pattern a 3x7, with 3 treatments and 7 repetitions of Bali beef. The treatments were: (P1) meat stored at room temperature (27°C-35°C) for less than 1 day (fresh meat), (P2) meat stored at 0°C-4°C for 1 day -2 days (cold meat), (P3) meat stored at a minimum temperature of -18°C with a storage time of 1-7 days (frozen meat). The variables observed in this study were the nutritional content of meat, namely water content, protein, fat, ash and carbohydrates as well as pathogenic bacterial contamination, namely Total Plate Count (TPC), Colliform and E-Colli. The results of this study showed that the nutritional content of water content and ash content in fresh, chilled and frozen meat had no significant effect. However, the protein content decreased significantly when the meat was frozen. The fat and carbohydrate content had the opposite result, namely, there was a significant increase when the meat was frozen. In terms of meat microbiological contamination on TPC, Coliform and E-colli variables, showed that frozen meat had the highest microbial population followed by fresh meat and cold meat had the lowest total pathogenic microbes.