{"title":"Regeneration of Plantlets from Leaf Derived Callus of Aerva lanata, Juss: A Medicinal Plant","authors":"Prakasha, K. Rave, Kabballi Balakrishn, G. Lokesh","doi":"10.3923/AJBKR.2012.143.146","DOIUrl":null,"url":null,"abstract":"Plantlets regeneration through callus has been achieved in medicinal plant Aerva lanata of Amaranthaceae. Callus obtained by culturing young leaf disks on Murashige and Skoog's (MS) medium with 1.0 mg L-1 2, 4.D (dichlorophenoxyacetic acid) was subcultured on MS medium contg. 2.0 mg L-1 6, Benzyl Amino Purine (BAP), produced 30-40 multiple shoots. Rooting was achieved on half or full strength MS medium with 0.5-0.1 mg L-1 Naphthalene Acetic Acid (NAA) in 14 days. Regenerated plantlets were acclimatized and transferred to soil were showed normal morphol. characteristics. Survival of transplants was 90-95% under green house condition. [on SciFinder(R)]","PeriodicalId":274901,"journal":{"name":"Asian Journal of Biotechnology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2012-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Asian Journal of Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3923/AJBKR.2012.143.146","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
Plantlets regeneration through callus has been achieved in medicinal plant Aerva lanata of Amaranthaceae. Callus obtained by culturing young leaf disks on Murashige and Skoog's (MS) medium with 1.0 mg L-1 2, 4.D (dichlorophenoxyacetic acid) was subcultured on MS medium contg. 2.0 mg L-1 6, Benzyl Amino Purine (BAP), produced 30-40 multiple shoots. Rooting was achieved on half or full strength MS medium with 0.5-0.1 mg L-1 Naphthalene Acetic Acid (NAA) in 14 days. Regenerated plantlets were acclimatized and transferred to soil were showed normal morphol. characteristics. Survival of transplants was 90-95% under green house condition. [on SciFinder(R)]
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