Electroporation of Canine MCT Cells and the Examination by Impedance Measurement

Abstract

This paper presents the electroporation of canine MCT cells by a low voltage in microfluidic system. We examined the electroporation efficiency with pulse conditions and correlated the results from the impedance measurement to the membrane openings. Dielectrophoretic force was applied to position the cell at a desired location. Temporary and permanent electroporation cells was discriminated by using a combination of Yo-Pro-1 and propidium iodide (PI) fluorescent dyes. From the experiment, we determined the appropriate condition for the reversible electroporation of the canine MCT cells to be 15 sets of $2.5 \mathrm{V}_{p}$, 20kHz frequency, and 50-cycle pulses. The condition yielded 50% efficiency for the reversible electroporation. In addition, the area of the cell-membrane pores could be quantitatively examined from the conductance measured without a cell and those with a cell before and after applying electroporation pulses.