Evaluation of Selected Bio-insecticides on Tuta absoluta and Liriomyza trifolii on Tomato in Open Fields in Tharaka Nithi County, Kenya

Ngugi Ceciliah Njoki, Mbaka Jesca Njeri, Hellen W. Muthengi
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Abstract

A field experiment to determine the effect of Azadirachtin, Bacillus thuringiensis, Pyrethrin + Garlic extract, and Petroleum mineral oil in managing the tomato leaf miner (Tuta absoluta) and serpentine leaf miner (Liriomyza trifolii) was conducted for two seasons between December 2016 and November 2017 at Chuka, Tharaka-Nithi County. Tomato variety, Kilele F1, was planted in the field to evaluate the effect of selected commercial products on T. absoluta and L. trifolii. The product included Azadirachtin 0.03%, Pyrethrin + Garlic extract, Bacillus thuringiensis var Kurstaki 5% w/w), and Petroleum mineral oil 98.8%. There were five treatments in a randomized complete block design (RCBD) in three replicates. There was an application of sterile water in the control experiment. There was a significant difference (P < 0.05) between the biocontrol products and the control. The Azadirachtin at 0.03% treatments had the least number of leaves damaged by T. absoluta (0.43; 1.67) in the control treatment (1.7; 5.27) in both seasons. In both seasons, there were significant differences (P < 0.05) between treatments in the number of leaflets damaged by L. trifolii. In season 1, the least damaged fruits per plant (1.33) were recorded in Azadirachtin at 0.03%, compared to the highest damage (5.0) in the untreated control. In the second season, the low fruit damage (0.67) was recorded in Petroleum mineral oil, and the highest (2.33) was in the untreated control. The study concluded that Azadirachtin 0.03%, Bacillus thuringiensis var Kurstaki 5% w/w, and Petroleum mineral oil were efficacious in managing T. absoluta and L. trifolii and be integrated into the existing tomato pest management strategies.
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肯尼亚Tharaka Nithi县露天番茄番茄枯斑土虱和三叶枯斑Liriomyza trifolii的生物杀虫剂选择评价
2016年12月至2017年11月,在塔拉卡-尼提县Chuka进行了为期两个季节的田间试验,以确定印印苦素、苏云金芽孢杆菌、除虫菊酯+大蒜提取物和石油矿物油对番茄叶螨(Tuta absoluta)和蛇纹叶螨(Liriomyza trifolii)的防治效果。以番茄品种“Kilele F1”为试验材料,评价了所选商品产品对绝对白僵菌和三叶黑僵菌的防治效果。其中印楝素0.03%,除虫菊酯+大蒜提取物,苏云金芽孢杆菌5% w/w,石油矿物油98.8%。随机完全区组设计(RCBD) 5个处理,3个重复。在对照实验中应用无菌水。生物防治制剂与对照间差异有统计学意义(P < 0.05)。0.03%的印楝素处理的枯叶数最少(0.43;对照组为1.67,对照组为1.7;5.27)。在两个季节中,不同处理间被三叶曲叶虫破坏的小叶数有显著差异(P < 0.05)。在第1季,印楝素浓度为0.03%时,单株损害果实最少(1.33个),而未处理的对照最高(5.0个)。第二季,石油矿物油对果实的危害最小(0.67),未处理的对照最高(2.33)。结果表明,0.03%的印楝素、5%的苏云金芽孢杆菌和5%的石油矿物油可有效地防治番茄枯叶枯蛾和三叶枯蛾,并可纳入现有的番茄害虫防治策略中。
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