{"title":"The establishment of detecting carbohydrate antigen 199 (CA199) with immunofluorescence chromatography","authors":"Jiakun Cao, Lin Xia, Sheng Tang","doi":"10.1109/ICARM.2017.8273172","DOIUrl":null,"url":null,"abstract":"This study was designed to develop a immunofluorescence chromatography kit for detection of CA199 in human serum, and to evaluate the method performance. The fluorescent polystyrene particle conjugated with the monoclonal antibody MIC03 was used as a fluorescent label. The monoclonal antibody MIC04 and mouse anti-rabbit IgG were immobilized on the nitrocellulose membrane as the test line and control line. The linear response rang of the method to detect the serum CA199 was 0.67–500U/ml. And the intra-and inter-assay coefficient were 6.08% and 8.59%. Total 1200 samples were tested by the immunofluorescence chromatography kit, and the overall coincidence rate was 92.56%. The positive coincidence rate was 91.08%, the negative coincidence rate was 94.09%, and the Kappa value surpassed 0.8, which indicated that there was no significant difference between the results of the immunofluorescence chromatography kit and Elisa kit. In conclusion, the immunofluorescence chromatography kit for assaying CA199 provided high sensitivity, rapid, simple operation, low cost, single-servings and can be extensively used for clinical operarions.","PeriodicalId":416846,"journal":{"name":"International Conference on Advanced Robotics and Mechatronics","volume":"77 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Conference on Advanced Robotics and Mechatronics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/ICARM.2017.8273172","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
This study was designed to develop a immunofluorescence chromatography kit for detection of CA199 in human serum, and to evaluate the method performance. The fluorescent polystyrene particle conjugated with the monoclonal antibody MIC03 was used as a fluorescent label. The monoclonal antibody MIC04 and mouse anti-rabbit IgG were immobilized on the nitrocellulose membrane as the test line and control line. The linear response rang of the method to detect the serum CA199 was 0.67–500U/ml. And the intra-and inter-assay coefficient were 6.08% and 8.59%. Total 1200 samples were tested by the immunofluorescence chromatography kit, and the overall coincidence rate was 92.56%. The positive coincidence rate was 91.08%, the negative coincidence rate was 94.09%, and the Kappa value surpassed 0.8, which indicated that there was no significant difference between the results of the immunofluorescence chromatography kit and Elisa kit. In conclusion, the immunofluorescence chromatography kit for assaying CA199 provided high sensitivity, rapid, simple operation, low cost, single-servings and can be extensively used for clinical operarions.