Direct sequencing of PCR products using the Maxam-Gilbert method

Stefan Stamm , Frank M. Longo
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引用次数: 4

Abstract

Direct sequencing of polymerase chain reaction (PCR) products by using the Maxam-Gilbert method is described. In this method, one of the primers is end labeled. Thus it is possible to sequence the reaction product directly following purification using this chemical method.

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使用Maxam-Gilbert法直接测序PCR产物
直接测序的聚合酶链反应(PCR)的产品,通过使用maxam -吉尔伯特方法描述。在这种方法中,其中一个引物被末端标记。因此,可以使用这种化学方法在纯化后直接对反应产物进行排序。
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