{"title":"Sialidase activity in rimantadine-resistant and -sensitive influenza A viruses.","authors":"A Garcia-Sastre, E Villar, C Hannoun, J A Cabezas","doi":"10.1159/000468733","DOIUrl":null,"url":null,"abstract":"<p><p>Rimantadine-resistant and -sensitive influenza A variants were assayed for their sialidase (neuraminidase, EC 3.2.1.18) activity. The kinetic parameters determined (pH optimum, stability against different pH values, thermal stability, activity on methylumbelliferyl-alpha-D-N-acetylneuraminic acid, N-acetylneuraminyl-lactose, fetuin and bovine submandibular gland mucin as substrates, Km with the former substrate, inhibition by two competitive inhibitors, and behavior towards amantadine) revealed the same results for both variants of the virus. Thus, it can be deduced that resistance to rimantadine does not influence the sialidase activity of influenza A virus.</p>","PeriodicalId":11933,"journal":{"name":"Enzyme","volume":"43 4","pages":"207-11"},"PeriodicalIF":0.0000,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000468733","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Enzyme","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1159/000468733","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
Rimantadine-resistant and -sensitive influenza A variants were assayed for their sialidase (neuraminidase, EC 3.2.1.18) activity. The kinetic parameters determined (pH optimum, stability against different pH values, thermal stability, activity on methylumbelliferyl-alpha-D-N-acetylneuraminic acid, N-acetylneuraminyl-lactose, fetuin and bovine submandibular gland mucin as substrates, Km with the former substrate, inhibition by two competitive inhibitors, and behavior towards amantadine) revealed the same results for both variants of the virus. Thus, it can be deduced that resistance to rimantadine does not influence the sialidase activity of influenza A virus.
对金刚乙胺耐药型和敏感型甲型流感变异体唾液酸酶(神经氨酸酶，EC 3.2.1.18)活性进行了检测。测定的动力学参数(pH值最佳、对不同pH值的稳定性、热稳定性、对甲伞草酰- α - d - n -乙酰神经氨酸、n -乙酰神经氨酸-乳糖、胎儿蛋白和牛颌下腺粘蛋白作为底物的活性、对前者底物的Km、对两种竞争性抑制剂的抑制作用以及对金刚烷胺的行为)表明，两种病毒变体的结果相同。由此可以推断，对金刚乙胺的抗性并不影响甲型流感病毒唾液酸酶的活性。
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