APLIKASI EKSTRAK JINTAN HITAM (Nigella sativa L.) SEBAGAI ZAT ANTIBAKTERI PADA KAIN KAPAS DENGAN VARIASI METODE

Abstract

bakteri dapat dipandang perlu untuk menambahkan zat antibakteri pada kain kapas. Pada penelitian ini, zat antibakteri yang diaplikasikan pada kain kapas adalah ekstrak jintan hitam. Langkah awal yang dilakukan adalah menentukan konsentrasi optimum ekstrak jintan hitam dengan variasi 10%, 20%, 30%, 40%, dan 50% untuk diaplikasikan pada kain kapas menggunakan cara rendam peras-pemanasawetan ( pad-dry-cure ). Setelah diperoleh konsentrasi optimum, ekstrak diaplikasikan pada kain kapas dengan beberapa variasi metode, yaitu (1) tanpa plasma lucutan korona dan asam sitrat, ABSTRACT One of the clothing materials that are widely used by community is cotton fabric, which is famous for its comfort. Unfortunately, cotton fabric is very susceptible to bacterial colonization which can cause skin problems. Therefore, it is deemed necessary to add an antibacterial agent to cotton fabric. In this study, the antibacterial agent applied to cotton fabric was black cumin extract. The first step was to determine the optimum concentration of black cumin extract with variations of 10%, 20%, 30%, 40%, and 50%, to be applied to cotton fabric using a pad-dry-cure method. After obtaining the optimum concentration, the extract was applied to cotton fabric with several variations of methods, namely (1) without corona discharge plasma and citric acid, (2) with corona discharge plasma only, (3) with citric acid only, and (4) with corona discharge plasma and citric acid. The antibacterial ability test of black cumin extract cotton fabric was carried out using the AATCC TM100-2019 method. The results showed that black cumin extract had the ability as an antibacterial agent on cotton fabric. Cotton fabric with extracts of 30%, 40%, and 50% resulted in the highest reduction percentage, which were respectively 96.6%; 97.0%; and 97.0%; against S. aureus; and 79.4%; 79.5%; and 79.5% against E. coli. The method that produced the highest reduction percentage was the combination of corona discharge plasma technology and citric acid, which resulted in an increase in the reduction percentage to 99.9% of S. aureus and E. coli at an extract concentration of 30%. This method also produces the highest durability against household washing. After 9 times of household washing, the percentage of reduction only decreased from 99.9±0.14% to 77.7±0.3% against S. aureus and 99,9±0.12% decreased to 75.1±0.4% against E. coli.